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Engineering fluorescent protein substrates for the AAA+ Lon protease

AAA+ proteases, such as Escherichia coli Lon, recognize protein substrates by binding to specific peptide degrons and then unfold and translocate the protein into an internal degradation chamber for proteolysis. For some AAA+ proteases, attaching specific degrons to the N- or C-terminus of green flu...

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Detalhes bibliográficos
Main Authors: Wohlever, Matthew L., Nager, Andrew R., Baker, Tania A., Sauer, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3716333/
https://ncbi.nlm.nih.gov/pubmed/23359718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzs105
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