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Engineering fluorescent protein substrates for the AAA+ Lon protease

AAA+ proteases, such as Escherichia coli Lon, recognize protein substrates by binding to specific peptide degrons and then unfold and translocate the protein into an internal degradation chamber for proteolysis. For some AAA+ proteases, attaching specific degrons to the N- or C-terminus of green flu...

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Bibliografiske detaljer
Main Authors: Wohlever, Matthew L., Nager, Andrew R., Baker, Tania A., Sauer, Robert T.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3716333/
https://ncbi.nlm.nih.gov/pubmed/23359718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzs105
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