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Structure-Guided Design of an Engineered Streptavidin with Reusability to Purify Streptavidin-Binding Peptide Tagged Proteins or Biotinylated Proteins

Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged recombinant proteins to be affinity purified using the streptavidin matrix without the need of biotinylation. The major limitation of this powerful technology is the requirement to use biotin to elute the SBP-tag...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wu, Sau-Ching, Wong, Sui-Lam
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3712923/
https://ncbi.nlm.nih.gov/pubmed/23874971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069530
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