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Structure-Guided Design of an Engineered Streptavidin with Reusability to Purify Streptavidin-Binding Peptide Tagged Proteins or Biotinylated Proteins

Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged recombinant proteins to be affinity purified using the streptavidin matrix without the need of biotinylation. The major limitation of this powerful technology is the requirement to use biotin to elute the SBP-tag...

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Bibliografiske detaljer
Main Authors: Wu, Sau-Ching, Wong, Sui-Lam
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3712923/
https://ncbi.nlm.nih.gov/pubmed/23874971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069530
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