Engineering of a Bacillus subtilis Strain with Adjustable Levels of Intracellular Biotin for Secretory Production of Functional Streptavidin

par Wu, Sau-Ching, Wong, Sui-Lam
Publié 2002

Structure-Guided Design of an Engineered Streptavidin with Reusability to Purify Streptavidin-Binding Peptide Tagged Proteins or Biotinylated Proteins

par Wu, Sau-Ching, Wong, Sui-Lam
Publié 2013

A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix

par Wu, Sau-Ching, Wang, Chris, Hansen, Dave, Wong, Sui-Lam
Publié dans Sci Rep (2017)

A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix

par Wu, Sau-Ching, Wang, Chris, Chin, Jonathan, Wong, Sui-Lam
Publié dans Sci Rep (2019)

Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High P...

par Fogen, Dawson, Wu, Sau-Ching, Ng, Kenneth Kai-Sing, Wong, Sui-Lam
Publié dans PLoS One (2015)

Enhanced Secretory Production of a Single-Chain Antibody Fragment from Bacillus subtilis by Coproduction of Molecular Chaperones

par Wu, Sau-Ching, Ye, Ruiqiong, Wu, Xu-Chu, Ng, Shi-Chung, Wong, Sui-Lam
Publié 1998

Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity

par Marangoni, Jesse M., Wu, Sau-Ching, Fogen, Dawson, Wong, Sui-Lam, Ng, Kenneth K. S.
Publié dans Sci Rep (2020)

The structure of the SBP-Tag–streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits

par Barrette-Ng, Isabelle H., Wu, Sau-Ching, Tjia, Wai-Mui, Wong, Sui-Lam, Ng, Kenneth K. S.
Publié 2013

Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from Bacillus subtilis: Effects of Molecular Chaperones and a Wall-Bound Protease on...

par Wu, Sau-Ching, Yeung, Jonathan C., Duan, Yanjun, Ye, Ruiqiong, Szarka, Steven J., Habibi, Hamid R., Wong, Sui-Lam
Publié 2002

Development of a LytE‐based high‐density surface display system in Bacillus subtilis

par Chen, Chyi‐Liang, Wu, Sau‐Ching, Tjia, Wai Mui, Wang, Christopher L. C., Lohka, Manfred J., Wong, Sui‐Lam
Publié 2008

Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin

par O'Sullivan, Valerie J., Barrette-Ng, Isabelle, Hommema, Eric, Hermanson, Greg T., Schofield, Mark, Wu, Sau-Ching, Honetschlaeger, Claudia, Ng, Kenneth K.-S., Wong, Sui-Lam
Publié 2012