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A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix

An engineered streptavidin, SAVSBPM18 with reversible biotin binding capability, has been successfully applied to purify biotinylated and streptavidin-binding peptide (SBP) tagged proteins. To simplify the preparation for the SAVSBPM18 affinity matrix without chemical conjugation, two bio-coupling a...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wu, Sau-Ching, Wang, Chris, Chin, Jonathan, Wong, Sui-Lam
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6399347/
https://ncbi.nlm.nih.gov/pubmed/30833609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-40044-4
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