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A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix

An engineered streptavidin, SAVSBPM18 with reversible biotin binding capability, has been successfully applied to purify biotinylated and streptavidin-binding peptide (SBP) tagged proteins. To simplify the preparation for the SAVSBPM18 affinity matrix without chemical conjugation, two bio-coupling a...

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Detaylı Bibliyografya
Yayımlandı:Sci Rep
Asıl Yazarlar: Wu, Sau-Ching, Wang, Chris, Chin, Jonathan, Wong, Sui-Lam
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group UK 2019
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6399347/
https://ncbi.nlm.nih.gov/pubmed/30833609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-40044-4
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