Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from Bacillus subtilis: Effects of Molecular Chaperones and a Wall-Bound Protease on Antibody Fragment Production

To develop an ideal blood clot imaging and targeting agent, a single-chain antibody (SCA) fragment based on a fibrin-specific monoclonal antibody, MH-1, was constructed and produced via secretion from Bacillus subtilis. Through a systematic study involving a series of B. subtilis strains, insufficie...

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Detalhes bibliográficos
Main Authors: Wu, Sau-Ching, Yeung, Jonathan C., Duan, Yanjun, Ye, Ruiqiong, Szarka, Steven J., Habibi, Hamid R., Wong, Sui-Lam
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2002
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126797/
https://ncbi.nlm.nih.gov/pubmed/12089002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.7.3261-3269.2002
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