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Structure-Guided Design of an Engineered Streptavidin with Reusability to Purify Streptavidin-Binding Peptide Tagged Proteins or Biotinylated Proteins

Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged recombinant proteins to be affinity purified using the streptavidin matrix without the need of biotinylation. The major limitation of this powerful technology is the requirement to use biotin to elute the SBP-tag...

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Sonraí Bibleagrafaíochta
Main Authors: Wu, Sau-Ching, Wong, Sui-Lam
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3712923/
https://ncbi.nlm.nih.gov/pubmed/23874971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069530
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