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A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification
As previously reported, a novel low temperature (LoTemp) polymerase chain reaction (PCR) catalyzed by a moderately heat-resistant (MHR) DNA polymerase with a chemical-assisted denaturation temperature set at 85 °C instead of the conventional 94–96 °C can achieve high-fidelity DNA amplification of a...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Molecular Diversity Preservation International (MDPI)
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3709816/ https://ncbi.nlm.nih.gov/pubmed/23787473 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms140612853 |
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