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A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification

As previously reported, a novel low temperature (LoTemp) polymerase chain reaction (PCR) catalyzed by a moderately heat-resistant (MHR) DNA polymerase with a chemical-assisted denaturation temperature set at 85 °C instead of the conventional 94–96 °C can achieve high-fidelity DNA amplification of a...

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Autori principali: Hong, Guofan, Lee, Sin Hang, Ge, Shichao, Zhou, Shaoxia
Natura: Artigo
Lingua:Inglês
Pubblicazione: Molecular Diversity Preservation International (MDPI) 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3709816/
https://ncbi.nlm.nih.gov/pubmed/23787473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms140612853
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