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A novel quantitative PCR mediated by high-fidelity DNA polymerase

The biggest challenge for accurate diagnosis of viral infectious disease is the high genetic variability of involved viruses, which affects amplification efficiency and results in low sensitivity and narrow spectrum. Here, we developed a new simple qPCR mediated by high-fidelity (HF) DNA polymerase....

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Zhang, Mengling, Liu, Kyle, Hu, Yihong, Lin, Yi, Li, Yang, Zhong, Ping, Jin, Xia, Zhu, Xiaoli, Zhang, Chiyu
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5583327/
https://ncbi.nlm.nih.gov/pubmed/28871131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-10782-4
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