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DNA damage reduces Taq DNA polymerase fidelity and PCR amplification efficiency.
DNA damage blocks DNA polymerase progression and increases miscoding. In this study we assessed the effects of specific lesions on Taq DNA polymerase fidelity and amplification efficiency. In the presence of 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG), Taq DNA polymerase inserted dCMP and to a les...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2007
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1945218/ https://ncbi.nlm.nih.gov/pubmed/17303074 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2007.01.169 |
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