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DNA damage reduces Taq DNA polymerase fidelity and PCR amplification efficiency.
DNA damage blocks DNA polymerase progression and increases miscoding. In this study we assessed the effects of specific lesions on Taq DNA polymerase fidelity and amplification efficiency. In the presence of 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG), Taq DNA polymerase inserted dCMP and to a les...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1945218/ https://ncbi.nlm.nih.gov/pubmed/17303074 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2007.01.169 |
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