A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

BACKGROUND AND OBJECTIVES: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3′ to 5′ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system...

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Bibliografische gegevens
Gepubliceerd in:Iran J Microbiol
Hoofdauteurs: Sankar, Prabu Siva, Citartan, Marimuthu, Siti, Aminah Ahmed, Skryabin, Boris V., Rozhdestvensky, Timofey S., Khor, Goot Heah, Tang, Thean Hock
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Tehran University of Medical Sciences 2019
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Original Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6635313/
https://ncbi.nlm.nih.gov/pubmed/31341574
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