A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

BACKGROUND AND OBJECTIVES: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3′ to 5′ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system...

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Detalhes bibliográficos
Publicado no:Iran J Microbiol
Main Authors: Sankar, Prabu Siva, Citartan, Marimuthu, Siti, Aminah Ahmed, Skryabin, Boris V., Rozhdestvensky, Timofey S., Khor, Goot Heah, Tang, Thean Hock
Formato: Artigo
Idioma:Inglês
Publicado em: Tehran University of Medical Sciences 2019
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6635313/
https://ncbi.nlm.nih.gov/pubmed/31341574
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