A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

BACKGROUND AND OBJECTIVES: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3′ to 5′ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system...

Cijeli opis

Spremljeno u:
Bibliografski detalji
Izdano u:Iran J Microbiol
Glavni autori: Sankar, Prabu Siva, Citartan, Marimuthu, Siti, Aminah Ahmed, Skryabin, Boris V., Rozhdestvensky, Timofey S., Khor, Goot Heah, Tang, Thean Hock
Format: Artigo
Jezik:Inglês
Izdano: Tehran University of Medical Sciences 2019
Teme:
Original Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6635313/
https://ncbi.nlm.nih.gov/pubmed/31341574
Oznake: Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!

Similar Items