A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

BACKGROUND AND OBJECTIVES: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3′ to 5′ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Iran J Microbiol
Autors principals: Sankar, Prabu Siva, Citartan, Marimuthu, Siti, Aminah Ahmed, Skryabin, Boris V., Rozhdestvensky, Timofey S., Khor, Goot Heah, Tang, Thean Hock
Format: Artigo
Idioma:Inglês
Publicat: Tehran University of Medical Sciences 2019
Matèries:
Original Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6635313/
https://ncbi.nlm.nih.gov/pubmed/31341574
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars