Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.

We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridizatio...

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Detalhes bibliográficos

Publicado no:	Mol Cell Biol
Principais autores:	Milbrandt, J D, Azizkhan, J C, Greisen, K S, Hamlin, J L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC370117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310370/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.3.7.1266-1273.1983
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