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Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.
We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridizatio...
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| 出版年: | Mol Cell Biol |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Taylor & Francis
1983
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC370117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310370/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.3.7.1266 |
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