Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.

We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridizatio...

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Bibliographic Details
Main Authors: Milbrandt, J D, Azizkhan, J C, Greisen, K S, Hamlin, J L
Format: Artigo
Language:Inglês
Published: 1983
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC370117/
https://ncbi.nlm.nih.gov/pubmed/6310370
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