Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.
We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridizatio...
Uloženo v:
| Vydáno v: | Mol Cell Biol |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Taylor & Francis
1983
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC370117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310370/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.3.7.1266-1273.1983 |
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