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Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.

We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridizatio...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Mol Cell Biol
Päätekijät: Milbrandt, J D, Azizkhan, J C, Greisen, K S, Hamlin, J L
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Taylor & Francis 1983
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC370117/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310370/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.3.7.1266
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