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Overproduction of dihydrofolate reductase and gene amplification in methotrexate-resistant Chinese hamster ovary cells.

Stable isolates of Chinese hamster ovary cells that are highly resistant to methotrexate have been selected in a multistep selection process. Quantitative immunoprecipitations have indicated that these isolates synthesize dihydrofolate reductase at an elevated rate over its synthesis in sensitive ce...

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Main Authors: Flintoff, W F, Weber, M K, Nagainis, C R, Essani, A K, Robertson, D, Salser, W
格式: Artigo
語言:Inglês
出版: 1982
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC369786/
https://ncbi.nlm.nih.gov/pubmed/6287233
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