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Amplification of a cloned Chinese hamster dihydrofolate reductase gene after transfer into a dihydrofolate reductase-deficient cell line.

We have transformed a dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary cell line to the DHFR+ phenotype with a recombinant cosmid (cH1) containing a functional Chinese hamster DHFR gene (J.D. Milbrandt et al., Mol. Cell. Biol. 3:1266-1273, 1983). After exposure of cells to successive i...

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Autors principals: Milbrandt, J D, Azizkhan, J C, Hamlin, J L
Format: Artigo
Idioma:Inglês
Publicat: 1983
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC370118/
https://ncbi.nlm.nih.gov/pubmed/6310371
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