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Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.

We isolated overlapping recombinant cosmids that represent the equivalent of two complete dihydrofolate reductase amplicon types from the methotrexate-resistant CHO cell line CHOC400. The type I amplicons are 260 kilobases long, are arranged in head-to-tail fashion, and represent 10 to 15% of the am...

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Bibliografski detalji
Glavni autori: Looney, J E, Hamlin, J L
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC365110/
https://ncbi.nlm.nih.gov/pubmed/3821723
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