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Enhancement of methotrexate resistance and dihydrofolate reductase gene amplification by treatment of mouse 3T6 cells with hydroxyurea.

We investigated various parameters associated with the initial selection of mouse 3T6 cells for resistance to single concentrations of methotrexate and characterized resistant colonies for the presence of additional (amplified) copies of the dihydrofolate reductase gene. Our results indicate that th...

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Autori principali: Brown, P C, Tlsty, T D, Schimke, R T
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1983
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC368639/
https://ncbi.nlm.nih.gov/pubmed/6877240
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