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In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR

BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage...

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Detalles Bibliográficos
Publicado en:BMC Clin Pathol
Main Authors: Quach, Nancy, Goodman, Myron F, Shibata, Darryl
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2004
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368439/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15028125/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1472-6890-4-1
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