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In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR

BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage...

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Hlavní autoři: Quach, Nancy, Goodman, Myron F, Shibata, Darryl
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC368439/
https://ncbi.nlm.nih.gov/pubmed/15028125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6890-4-1
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