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In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR
BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2004
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC368439/ https://ncbi.nlm.nih.gov/pubmed/15028125 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6890-4-1 |
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