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In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR

BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage...

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書目詳細資料
發表在:BMC Clin Pathol
Main Authors: Quach, Nancy, Goodman, Myron F, Shibata, Darryl
格式: Artigo
語言:Inglês
出版: BMC 2004
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368439/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15028125/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1472-6890-4-1
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