Qualitative and quantitative characterization of protein-phosphoinositide interactions with liposome-based methods

We characterized phosphoinositide binding of the S. cerevisiae PROPPIN Hsv2 qualitatively with density flotation assays and quantitatively through isothermal titration calorimetry (ITC) measurements using liposomes. We discuss the design of these experiments and show with liposome flotation assays t...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Busse, Ricarda A., Scacioc, Andreea, Hernandez, Javier M., Krick, Roswitha, Stephan, Milena, Janshoff, Andreas, Thumm, Michael, Kühnel, Karin
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Landes Bioscience 2013
Pynciau:
Protocol
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3669185/
https://ncbi.nlm.nih.gov/pubmed/23445924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/auto.23978
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