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Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4

Alanine racemase (Alr(MB4)), a dimeric PLP-dependent thermostable enzyme from the anaerobic eubacterium Thermoanaerobacter tengcongensis MB4, was expressed and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion...

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Autors principals: Dong, Hui, Xu, Shujing, Lu, Xiaoyun, He, Guangzheng, Zhao, Ranran, Chen, Shuai, Fu, Sheng, Ju, Jiansong
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668588/
https://ncbi.nlm.nih.gov/pubmed/23722847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113011743
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