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Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4
Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffus...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2635857/ https://ncbi.nlm.nih.gov/pubmed/19194012 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910900013X |
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