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Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1
Biosynthetic alanine racemase (Alr(PA)) from Pseudomonas aeruginosa PAO1 carrying a His(6) tag was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni(2+)-chelating affinity and anion-exchange chromatography for X-ray crystallographic analysis. Crystals were grown by the hanging-drop v...
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| Publicado no: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4259224/ https://ncbi.nlm.nih.gov/pubmed/25484210 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14022432 |
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