Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffus...

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Bibliographic Details
Main Authors: Ju, Jiansong, Qi, Jianxun, Xu, Shujing, Ohnishi, Kouhei, Benedik, Michael J., Xue, Yanfen, Ma, Yanhe
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2009
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635857/
https://ncbi.nlm.nih.gov/pubmed/19194012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910900013X
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