Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically d...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Qi, Lei S., Larson, Matthew H., Gilbert, Luke A., Doudna, Jennifer A., Weissman, Jonathan S., Arkin, Adam P., Lim, Wendell A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2013
Soggetti:
Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664290/
https://ncbi.nlm.nih.gov/pubmed/23452860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2013.02.022
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi