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Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically d...

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Bibliografische gegevens
Hoofdauteurs: Qi, Lei S., Larson, Matthew H., Gilbert, Luke A., Doudna, Jennifer A., Weissman, Jonathan S., Arkin, Adam P., Lim, Wendell A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2013
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664290/
https://ncbi.nlm.nih.gov/pubmed/23452860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2013.02.022
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