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Selection for intrabody solubility in mammalian cells using GFP fusions
Single-chain antibody fragments (scFv) expressed in the cytoplasm of mammalian cells, also called intrabodies, have many applications in functional proteomics. These applications are however limited by the aggregation-prone behaviour of many intrabodies. We show here that two scFv with highly homolo...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3655372/ https://ncbi.nlm.nih.gov/pubmed/21997307 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr049 |
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