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Physico-chemical determinants of soluble intrabody expression in mammalian cell cytoplasm

Soluble antibody fragments are desirable not only as potential therapeutic and diagnostic agents for extracellular targets but also as ‘intrabodies’ for functional genomics, proteomics and gene therapy inside cells. However, antibody fragments are notoriously aggregation-prone when expressed intrace...

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Bibliografiske detaljer
Main Authors: Kvam, Erik, Sierks, Michael R., Shoemaker, Charles B., Messer, Anne
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865363/
https://ncbi.nlm.nih.gov/pubmed/20378699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzq022
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