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Intrabody expression in eukaryotic cells
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector we describe the cloning step into a mammalian expression vector, the transient transfection of HeLa cell line and the monitoring of intrabody expression by immu...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Humana Press
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3194088/ https://ncbi.nlm.nih.gov/pubmed/19554297 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-302-2_15 |
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