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Expression of single-chain Fv fragments in E. coli cytoplasm
The most frequently used approach to produce scFv and Fab in E. coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished either by using a specially engineered E. coli strains...
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| Asıl Yazarlar: | , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Humana Press
2009
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3101525/ https://ncbi.nlm.nih.gov/pubmed/19554299 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-302-2_17 |
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