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Expression of single-chain Fv fragments in E. coli cytoplasm
The most frequently used approach to produce scFv and Fab in E. coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished either by using a specially engineered E. coli strains...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Humana Press
2009
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3101525/ https://ncbi.nlm.nih.gov/pubmed/19554299 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-302-2_17 |
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