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Use of a Novel 5′-Regioselective Phosphitylating Reagent for One-Pot Synthesis of Nucleoside 5′-Triphosphates from Unprotected Nucleosides

The 5′-triphosphates are the building blocks for the enzymatic synthesis of DNAs and RNAs. This unit presents a protocol for the convenient synthesis of 2′-deoxyribo- and ribo-nucleoside 5′-triphosphates (dNTPs and NTPs) containing all the natural bases and the modified bases. This one-pot synthesis...

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書誌詳細
主要な著者: Caton-Williams, Julianne, Hoxhaj, Rudiona, Fiaz, Bilal, Huang, Zhen
フォーマット: Artigo
言語:Inglês
出版事項: 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3655200/
https://ncbi.nlm.nih.gov/pubmed/23512692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142700.nc0130s52
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