Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.

To examine the stability of yeast (Saccharomyces cerevisiae) pre-mRNA structures, we inserted a series of small sequence elements that generated potential RNA hairpins at the 5' splice site and branch point regions. We analyzed spliceosome assembly and splicing in vitro as well as splicing and nucle...

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Pubblicato in:	Mol Cell Biol
Autori principali:	Goguel, V, Wang, Y, Rosbash, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Taylor & Francis 1993
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC364746/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8413277/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.11.6841-6848.1993
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