Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.

To examine the stability of yeast (Saccharomyces cerevisiae) pre-mRNA structures, we inserted a series of small sequence elements that generated potential RNA hairpins at the 5' splice site and branch point regions. We analyzed spliceosome assembly and splicing in vitro as well as splicing and nucle...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Goguel, V, Wang, Y, Rosbash, M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC364746/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8413277/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.11.6841-6848.1993
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