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Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

We show that comprehensive sequence-function maps obtained by deep sequencing can be used to reprogram interaction specificity and to leapfrog over bottlenecks in affinity maturation by combining many individually small contributions not detectable in conventional approaches. We use this approach to...

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Detalhes bibliográficos
Main Authors: Whitehead, Timothy A, Chevalier, Aaron, Song, Yifan, Dreyfus, Cyrille, Fleishman, Sarel J, De Mattos, Cecilia, Myers, Chris A, Kamisetty, Hetunandan, Blair, Patrick, Wilson, Ian A, Baker, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3638900/
https://ncbi.nlm.nih.gov/pubmed/22634563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2214
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