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Assessing mRNA Translation: Deep Sequencing of Ribosome Footprints

A translating ribosome protects a discrete fragment of ∼30 nt on its mRNA template from nuclease digestion [Steitz, JA, (1969) Nature 224, 957]. Ribosome profiling determines the positions of active ribosomes on cellular mRNA by deep sequencing the ribosome-protected mRNA fragments [Ingolia, NT et a...

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Bibliografski detalji
Glavni autori: Vaidyanathan, Ramesh, Kuersten, Scott, Radek, Agnes, Swami, Sajani, Syed, Fraz
Format: Artigo
Jezik:Inglês
Izdano: Association of Biomolecular Resource Facilities 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635272/
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