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Eliminating Ultracentrifugation in the Deep Sequencing of Ribosome-Protected mRNA Fragments Using Polysomes and Monosomes

RNA abundance measured either by microarrays or high-throughput sequencing reflects protein levels for transcripts that are not subjected to translation control. Isolation of translating ribosomes (polysomes and monosomes) and analysis of the associated mRNAs provide a better measure of translation...

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Main Authors: Kuersten, Scott, Radek, Agnes, Swami, Sajani, Rouskin, Silvi, Dunn, Joshua, Weissman, Jonathan, Vaidyanathan, Ramesh, Baas, Brad
格式: Artigo
語言:Inglês
出版: Association of Biomolecular Resource Facilities 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3630598/
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