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Eliminating Ultracentrifugation in the Deep Sequencing of Ribosome-Protected mRNA Fragments Using Polysomes and Monosomes
RNA abundance measured either by microarrays or high-throughput sequencing reflects protein levels for transcripts that are not subjected to translation control. Isolation of translating ribosomes (polysomes and monosomes) and analysis of the associated mRNAs provide a better measure of translation...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Association of Biomolecular Resource Facilities
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3630598/ |
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