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The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments

Recent studies highlight the importance of translational control in determining protein abundance, underscoring the value of measuring gene expression at the level of translation. We present a protocol for genome-wide, quantitative analysis of in vivo translation by deep sequencing. This ribosome pr...

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Bibliografski detalji
Glavni autori: Ingolia, Nicholas T., Brar, Gloria A., Rouskin, Silvia, McGeachy, Anna M., Weissman, Jonathan S.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3535016/
https://ncbi.nlm.nih.gov/pubmed/22836135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.086
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