Modular assembly of transposon integratable multigene vectors using RecWay assembly

Studying complex biological processes such as cancer development, stem cell induction and transdifferentiation requires the modulation of multiple genes or pathways at one time in a single cell. Herein, we describe straightforward methods for rapid and efficient assembly of bacterial marker free mul...

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Detalles Bibliográficos
Autores principales: Moriarity, Branden S., Rahrmann, Eric P., Keng, Vincent W., Manlove, Luke S., Beckmann, Dominic A., Wolf, Natalie K., Khurshid, Touba, Bell, Jason B., Largaespada, David A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632113/
https://ncbi.nlm.nih.gov/pubmed/23444141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt115
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