Modular assembly of transposon integratable multigene vectors using RecWay assembly

Studying complex biological processes such as cancer development, stem cell induction and transdifferentiation requires the modulation of multiple genes or pathways at one time in a single cell. Herein, we describe straightforward methods for rapid and efficient assembly of bacterial marker free mul...

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Hlavní autoři: Moriarity, Branden S., Rahrmann, Eric P., Keng, Vincent W., Manlove, Luke S., Beckmann, Dominic A., Wolf, Natalie K., Khurshid, Touba, Bell, Jason B., Largaespada, David A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2013
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632113/
https://ncbi.nlm.nih.gov/pubmed/23444141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt115
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