Modular assembly of transposon integratable multigene vectors using RecWay assembly

Studying complex biological processes such as cancer development, stem cell induction and transdifferentiation requires the modulation of multiple genes or pathways at one time in a single cell. Herein, we describe straightforward methods for rapid and efficient assembly of bacterial marker free mul...

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Autors principals: Moriarity, Branden S., Rahrmann, Eric P., Keng, Vincent W., Manlove, Luke S., Beckmann, Dominic A., Wolf, Natalie K., Khurshid, Touba, Bell, Jason B., Largaespada, David A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2013
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632113/
https://ncbi.nlm.nih.gov/pubmed/23444141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt115
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