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Modular assembly of transposon integratable multigene vectors using RecWay assembly

Studying complex biological processes such as cancer development, stem cell induction and transdifferentiation requires the modulation of multiple genes or pathways at one time in a single cell. Herein, we describe straightforward methods for rapid and efficient assembly of bacterial marker free mul...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Moriarity, Branden S., Rahrmann, Eric P., Keng, Vincent W., Manlove, Luke S., Beckmann, Dominic A., Wolf, Natalie K., Khurshid, Touba, Bell, Jason B., Largaespada, David A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632113/
https://ncbi.nlm.nih.gov/pubmed/23444141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt115
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