Modular assembly of transposon integratable multigene vectors using RecWay assembly

Studying complex biological processes such as cancer development, stem cell induction and transdifferentiation requires the modulation of multiple genes or pathways at one time in a single cell. Herein, we describe straightforward methods for rapid and efficient assembly of bacterial marker free mul...

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Detalhes bibliográficos
Main Authors: Moriarity, Branden S., Rahrmann, Eric P., Keng, Vincent W., Manlove, Luke S., Beckmann, Dominic A., Wolf, Natalie K., Khurshid, Touba, Bell, Jason B., Largaespada, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632113/
https://ncbi.nlm.nih.gov/pubmed/23444141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt115
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