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Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox

Interfering with disulfide bond formation impedes protein folding and promotes endoplasmic reticulum (ER) stress. Due to limitations in measurement techniques, the relationships of altered thiol redox and ER stress have been difficult to assess. We report that fluorescent lifetime measurements circu...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Avezov, Edward, Cross, Benedict C.S., Kaminski Schierle, Gabriele S., Winters, Mikael, Harding, Heather P., Melo, Eduardo Pinho, Kaminski, Clemens F., Ron, David
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Rockefeller University Press 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3628511/
https://ncbi.nlm.nih.gov/pubmed/23589496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201211155
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