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E6AP/UBE3A Ubiquitin Ligase Harbors Two E2∼ubiquitin Binding Sites
By exploiting (125)I-polyubiquitin chain formation as a functional readout of enzyme activity, we have quantitatively examined the mechanism of human E6AP/UBE3A for the first time. Initial rate studies identify UbcH7 as the cognate E2 carrier protein for E6AP, although related Ubc5 isoforms and the...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3624418/ https://ncbi.nlm.nih.gov/pubmed/23439649 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.458059 |
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