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In silico modeling of the cryptic E2∼ubiquitin–binding site of E6-associated protein (E6AP)/UBE3A reveals the mechanism of polyubiquitin chain assembly

To understand the mechanism for assembly of Lys(48)-linked polyubiquitin degradation signals, we previously demonstrated that the E6AP/UBE3A ligase harbors two functionally distinct E2∼ubiquitin–binding sites: a high-affinity Site 1 required for E6AP Cys(820)∼ubiquitin thioester formation and a cano...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Ronchi, Virginia P., Kim, Elizabeth D., Summa, Christopher M., Klein, Jennifer M., Haas, Arthur L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5672028/
https://ncbi.nlm.nih.gov/pubmed/28924046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.813477
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