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E6AP/UBE3A Ubiquitin Ligase Harbors Two E2∼ubiquitin Binding Sites

By exploiting (125)I-polyubiquitin chain formation as a functional readout of enzyme activity, we have quantitatively examined the mechanism of human E6AP/UBE3A for the first time. Initial rate studies identify UbcH7 as the cognate E2 carrier protein for E6AP, although related Ubc5 isoforms and the...

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Bibliografische gegevens
Hoofdauteurs: Ronchi, Virginia P., Klein, Jennifer M., Haas, Arthur L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Biochemistry and Molecular Biology 2013
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3624418/
https://ncbi.nlm.nih.gov/pubmed/23439649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.458059
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