RNA–protein analysis using a conditional CRISPR nuclease

RNA-binding proteins control the fate and function of the transcriptome in all cells. Here we present technology for isolating RNA–protein partners efficiently and accurately using an engineered clustered regularly interspaced short palindromic repeats (CRISPR) endoribonuclease. An inactive version...

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Bibliografske podrobnosti
Main Authors: Lee, Ho Young, Haurwitz, Rachel E., Apffel, Alex, Zhou, Kaihong, Smart, Brian, Wenger, Craig D., Laderman, Stephen, Bruhn, Laurakay, Doudna, Jennifer A.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2013
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3619310/
https://ncbi.nlm.nih.gov/pubmed/23493562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1302807110
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