RNA–protein analysis using a conditional CRISPR nuclease

RNA-binding proteins control the fate and function of the transcriptome in all cells. Here we present technology for isolating RNA–protein partners efficiently and accurately using an engineered clustered regularly interspaced short palindromic repeats (CRISPR) endoribonuclease. An inactive version...

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Detalhes bibliográficos
Main Authors: Lee, Ho Young, Haurwitz, Rachel E., Apffel, Alex, Zhou, Kaihong, Smart, Brian, Wenger, Craig D., Laderman, Stephen, Bruhn, Laurakay, Doudna, Jennifer A.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3619310/
https://ncbi.nlm.nih.gov/pubmed/23493562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1302807110
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