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Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...

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Bibliografiske detaljer
Main Authors: Maco, Bohumil, Holtmaat, Anthony, Cantoni, Marco, Kreshuk, Anna, Straehle, Christoph N., Hamprecht, Fred A., Knott, Graham W.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3585404/
https://ncbi.nlm.nih.gov/pubmed/23468982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0057405
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